Direct blood analysis of Bartonella bacilliformis Multi Locus Sequence Typing in patients with Oroya’s fever during a Peruvian outbreak

The bacteria Bartonella bacilliformis is the etiological agent of Carrion’s disease, which is a neglected poverty-related disease, affecting Mountain Andean valleys of Peru, Colombia and Ecuador. This disease, in absence of treatment presents a high mortality during the acute phase, called Oroya’s Fever. The second phase is characterized by the development of dermal eruptions, known as “Peruvian wart”. This bacterium is a fastidious slow growing microorganism, being difficult and cumbersome to culture and isolate from clinical sources. Then, the available data about phylogenetic relationship in clinical samples are really scarce, but suggesting high variability. The aim of the study was to perform direct blood analysis of B. bacilliformis Multi Locus Sequence Typing (MLST), a genotyping tool, in patients with Oroya fever during an outbreak. The present study demonstrate that the direct blood MLST PCR is a technique useful in the phylogenic characterization of this fastidious microorganism endemic from Andean regions. In this study, we demonstrate that the outbreak of Oroya’s fever was caused by closely related Sequence Typing (ST) microorganisms and, additionally, new STs have been described.

Author(s): Pons, Maria J.; Silva, Wilmer; Gomes, Cláudia; Ruiz, Joaquim; del Valle Mendoza, Juana Mercedes
Source: Universidad Peruana de Ciencias Aplicadas - UPC

URL: http://hdl.handle.net/10757/582375

Evaluación in vitro del efecto antibacteriano y citotóxico del extracto metanólico de semilla y pulpa de la myrciaria dubia (camu camu) sobre cepas de streptococcus mutans (ATCC 25175) y streptococcus sanguinis (ATCC 10556)

Objective: The aim of this study was to evaluate the antibacterial and cytotoxic effect of the methanol extract of the seed and pulp of Myrciaria dubia (camu camu) against Streptococcus mutans (ATCC 25175) and Streptococcus sanguinis (ATCC 10556). Materials and Methods: For the present in vitro experimental study two methanolic extracts were prepared from the seed and pulp of Myrciaria dubia (camu camu) to be tested against Streptococcus mutans and Streptococcus sanguinis. Ten independents tests were prepared for each type of extract, using Clorhexidine at 0.12% solution as a positive control. The method used was agar diffusion test preparing wells with the experimental solutions cultivated in anaerobic conditions for 48 hours at 37°C, after this time the growth inhibition halo was analysed in millimeters using a vernier. Meanwhile, the Minimum Inhibitory Concentration (MIC) and the cytotoxic effect (CC50) over Jurkat T cell line was found. Results: The methanolic seed extract had more antibacterial effect over Streptococcus mutans with an inhibitory halo of 21.36mm ± 6.35, while the pulp had 16.20mm ± 2.08. For the Streptococcus sanguinis group the inhibitory halo were 19.21mm ± 5.18 and 19.34mm ± 2.90 for the methanolic seed and pulp extract respectively. The MIC for the pulp extract was 125µg/ml for both strains, whereas for the seed antibacterial activity was observed even at low concentrations. Finally the CC50 for the seed extract was at a higher concentration than 800 µg/ml and 524.37µg/ml for the pulp exactract. Conclusions: The methanolic seed and pulp extract of Myrciaria dubia (camu camu) had antibacterial effect against Streptococcus mutans and Streptococcus sanguinis. These extracts were not cytotoxic.

Author: Camere Colarossi, Rosella Vanina

URL: http://hdl.handle.net/10757/581744

EPUB: http://repositorioacademico.upc.edu.pe/upc/bitstream/10757/581744/2/CAMERE_cm.epub

Prevalencia de caries de aparición temprana en niños de 3 a 5 años y 11 meses de edad y su asociación con sus factores predisponentes en el policlínico Essalud Chincha de setiembre 2012 a febrero 2013

Objective: To determine the prevalence of early childhood caries (ECC) in children aged 3-5 years and 11 months old and its association with predisposing factors at the Polyclinic EsSalud Chincha since September 2012 to February 2013. Materials and Methods: It was a descriptive, observational, cross-sectional study. The information was collected from the office files in the Polyclinic Essalud Chincha - Peru. The sample consisted in 300 Medical Files of children aged 3-5 years that have been attended during September 2012 to February 2013. Variables of gender, age, oral hygiene index (OHI), affected surfaces and carbohydrate intake frequency (CIF) were evaluated. Microsoft Excel and SPSS statistical package was used. Prevalence data was obtained and the association was determined by testing chi square with a level of statistical significance (p <0.05). Results: The prevalence of ECC was 67.33%. Female gender showed a prevalence of 50.4% and male gender of 49.6%. It was found a greater average of dental caries in the occlusal surface (2.29), then the interproximal surface (0.78) and finally the smooth surface (0.55). Children aged 4 years had a higher prevalence of CAT (38.2%). Statistically significant association between ECC and variables such as age (p = 0.005), frequency of sugar consumption (p = 0.000) and soft plaque index (p = 0.000) was found. Conclusions: A high prevalence of ECC was found in children. The predisposing factors for the presence of caries were age, oral hygiene and sugar intake.
Author: Tovar Zevallos, Manuel AlbertoURL: http://hdl.handle.net/10757/581686
EPUB: http://repositorioacademico.upc.edu.pe/upc/bitstream/10757/581686/2/TOVAR_ZM_cybertesis.epub

Propuesta de un modelo de sistema de gestión de la seguridad de la información en una pyme basado en la norma ISO/IEC 27001

This Project “Implementation Proposal of an Information Security Management System (ISMS) in an SME, based on the ISO 27001 standard” propose an implementation model of a Information Security Management System (ISMS) in a SME, in order to obtain the ISO 27001 certification in a simple way, at low cost and reducing implementation periods. For the development of this project, the project team make an exhaustive analysis of the ISO/IEC 27000 family of standards, in order to identify the minimum needed requirements for the implementation of an ISMS in a SME. Based on the analyzed, the project team design an ISMS model that allows their subsequent implementation in a SME. In addition, the project team develop the implementation procedure of the model, which allow an SME to implement the model by their own hands. Finally, the application of the proposed model is performed, following the established methodology, in an SME, to proof their viability and provide an implementation example for future project stakeholders. The long-term goal of this Project is to facilitate the implementation of an ISMS for SMEs on their own, to distinguish itself from competition or some other interest.

Authors: Berríos Mesía, César Augusto; Rocha Cam, Martín Augusto

URL: http://hdl.handle.net/10757/581891

EPUB: http://repositorioacademico.upc.edu.pe/upc/bitstream/10757/581891/2/berrios_mc-rocha_cm.epub

Propuesta de mejora en la metodología para la gestión de servicios TI en Intercorp

El presente proyecto propone el estudio de la arquitectura empresarial actual (AS-IS) y futuro (TO-BE) del proceso de servicios compartidos del grupo Intercorp. También se estudia la gestión de servicios de tecnologís de la información (TI) a realizar dentro de dicho proceso de servicios compartidos y la identificación de dos nuevos servicios que permitirán la aplicación de ITIL como modelo o estándar de referencia. Una vez identificados los servicios, se analizará el ciclo de vida de ambos como proceso y se estudiará y propondrá el uso de una metodología predictiva o ágil y su integración con ITIL. El objetivo principal se centra en proponer mejoras en el proceso de servicios compartidos, mediante el uso de buenas prácticas e indicadores de gestión, usando el enfoque de arquitectura empresarial en base al modelo de TOGAF, gestionando los servicios TI mediante ITIL y generando el ciclo de vida de los procesos sobre la base de la norma ISO_IEC_12207, permitiendo que la gerencia de Servicios Compartidos pueda soportar y gestionar eficientemente los proyectos de la empresa para generar mayor valor a la corporación y así mantenerla alineada en mayor medida a sus objetivos estratégicos.

Authors: Huamaní Portocarrero, Angélica Isabel; Cóndor Colqui, Jimmy Frank

URL: http://hdl.handle.net/10757/581903

EPUB: http://repositorioacademico.upc.edu.pe/upc/bitstream/10757/581903/2/PAP+XIV+-+GRUPO+10+TRABAJO+FINAL+PAP.epub