Abstract
Rationale: It has been reported that PN (parenteral
nutrition) in preterm neonates may have deleterious
effects on hepatic function. We also studied the influence
of peroxidation in PN bags, with and without light
protection.
Methods: 30 neonates weighing 1000 1500 g on TPN were
studied prospectively. Serum samples were taken at start
and at end of PN. Peroxides from 31 bags at 0, 5, 18 and 24
hours were measured. PN samples were taken from both
light protected and not light protected PN bags.
Results: Patients: (1) With PN: GOT = 28.63±13.00 IU;
GPT = 7.37±5.10 IU; TB = 9.03±3.40 IU; DB = 0.845
±0.43 UI; GGT = 110.41±81.87 IU. (2) Without PN: GOT =
28.73±16.36 IU; GPT = 10.53±8.38 IU; TB = 6.36±3.91 IU;
DB = 1.35±1.53 IU; GGT = 128.38±75.74 IU. Significant
differences: GPT, TB and DB (p < 0.05).
Peroxides in bags: light protected and not, respectively,
at 0 hours: 278.01±139.92 and 299.87±166.00, at
5 hours: 142.28±117.93 and 155.11±140.81, at 18 hours
183.39±115.40 and 212.92±133.72 and at 24 hours 258.58±187.81 and 284.55±162.78. At 18 hours the
difference was significant (p < 0.05).
Conclusions:
1. TB and 18-hour hydroperoxides concentrations were
higher in serum, with PN and in light unprotected PN
bags, respectively.
2. GPT and DB serum levels were lower with PN.
3. Within the conditions of this study, no association
was found between hepatic function alterations and
short-term TPN as well as with bag light exposure in
neonates.
Autor: Ferreyra, M.E.; Ocaña, M.C.; Bullón, E.N.
Fuente: 31st ESPEN Congress Vienna, Austria
29 August–1 September 2009
URL: http://hdl.handle.net/10757/347040
miércoles, 1 de abril de 2015
Bilateral endogenous ophthalmitis due to Candida glabrata after complicated bariatric surgery
Abstract
Case report: A 43-year-old female presented with decreased visual acuity in the right eye.“Snowball-like” retinal lesions were found in both eyes on examination. Due to a lackof improvement with intravitreal antifungal empirical treatment, vitreous culture wasperformed and Candida glabrata was isolated. The patient then received intravitreal ampho-tericin B, as well as systemic treatment with caspofungin and amphotericin B lipid complex.Discussion: Endogenous fungal endophthalmitis is a sight-threatening condition. There arefew reports of C. glabrata endogenous endophthalmitis. Treatment regimens for Candidaendophthalmitis include combinations of systemic and/or intravitreal antifungals, as wellas vitrectomy.
Autor: Pizango, O.; Tejeda, E.; Buendia, M.; Lujana, S.
Fuente: Archivos de la Sociedad Española de Oftalmología
Case report: A 43-year-old female presented with decreased visual acuity in the right eye.“Snowball-like” retinal lesions were found in both eyes on examination. Due to a lackof improvement with intravitreal antifungal empirical treatment, vitreous culture wasperformed and Candida glabrata was isolated. The patient then received intravitreal ampho-tericin B, as well as systemic treatment with caspofungin and amphotericin B lipid complex.Discussion: Endogenous fungal endophthalmitis is a sight-threatening condition. There arefew reports of C. glabrata endogenous endophthalmitis. Treatment regimens for Candidaendophthalmitis include combinations of systemic and/or intravitreal antifungals, as wellas vitrectomy.
Autor: Pizango, O.; Tejeda, E.; Buendia, M.; Lujana, S.
Fuente: Archivos de la Sociedad Española de Oftalmología
Misdiagnosed outbreak of bartonella bacilliformis in Peruvian Amazon department
Abstract
Background: In March 2013, the presence of an outbreak of Bartonella bacilliformis in the Rodriguez de Mendoza (Amazonas department, Peru) was reported. B. bacilliformis is an endemic pathogen of the Andean region, responsible for Carrion’s disease. One of the main problems of this illness is the lack of adequate technical and human resources for proper diagnosis in endemic rural areas. The objective of this study was to characterize a supposed B. bacilliformis outbreak, internationally informed in Rodriguez de Mendoza province. Methods & Materials: Fifty-three blood samples were recovered from people diagnosed with Carrion’s disease, either by optical microscopy and/or clinical manifestations. In all cases epidemiological and clinical data were recorded. The samples were cultured on Columbia Agar adding 10% of sheep blood and incubated at 28 ◦C for a period of 10 weeks. Every 14 days the plates were visually inspected to detect any bacterial growth. Additionally, the DNA was directly extracted from blood and 2 different 16S rRNA PCR schemes were used, one specific for Bartonella genus and other using universal primers. Twenty-six amplified products of universal 16S rRNA were randomly recovered and sequenced. Results: The main clinical presentations reported were headache (51%), physical discomfort (51%), chill (32%) and fever (24, 5%). Only 3 blood cultures were positive. No positive PCR was obtained when using the Bartonella specific PCR either on blood or on cultured bacteria. However, all the PCR with the universal primers were positive. The sequenced 26 (49%) samples were identified as Sphingomonas spp. being this microorganism the causative agent of this outbreak. In 17% of the cases, patients were reported to have aquatic activities. Conclusion: Several Sphingomonas spp. infections in humans have been reported, mostly limited to sporadic case reports or intra-hospitalary outbreaks, but as far as we know this is the first outbreak of Sphingomonas spp. described in a non-hospital environment. The association between 17% of patients with aquatic activities suggests that this was the most feasible transmission way. Training of health staff and development of new diagnostic able to be implemented in rural endemic areas is urgent in order to overcome wrong diagnostics and avoid wrong treatments.
Autor: Cornejo, A.; Casabona, V.; Gomes, C.S.P.; Tinco, C.; Martinez Pucho, S.; Suarez, L.; Ruiz, J.; Del Valle Mendoza, Juana
Fuente: 16th ICID Abstracts / International Journal of Infectious Diseases 21S (2014)
URL: http://hdl.handle.net/10757/347058
Background: In March 2013, the presence of an outbreak of Bartonella bacilliformis in the Rodriguez de Mendoza (Amazonas department, Peru) was reported. B. bacilliformis is an endemic pathogen of the Andean region, responsible for Carrion’s disease. One of the main problems of this illness is the lack of adequate technical and human resources for proper diagnosis in endemic rural areas. The objective of this study was to characterize a supposed B. bacilliformis outbreak, internationally informed in Rodriguez de Mendoza province. Methods & Materials: Fifty-three blood samples were recovered from people diagnosed with Carrion’s disease, either by optical microscopy and/or clinical manifestations. In all cases epidemiological and clinical data were recorded. The samples were cultured on Columbia Agar adding 10% of sheep blood and incubated at 28 ◦C for a period of 10 weeks. Every 14 days the plates were visually inspected to detect any bacterial growth. Additionally, the DNA was directly extracted from blood and 2 different 16S rRNA PCR schemes were used, one specific for Bartonella genus and other using universal primers. Twenty-six amplified products of universal 16S rRNA were randomly recovered and sequenced. Results: The main clinical presentations reported were headache (51%), physical discomfort (51%), chill (32%) and fever (24, 5%). Only 3 blood cultures were positive. No positive PCR was obtained when using the Bartonella specific PCR either on blood or on cultured bacteria. However, all the PCR with the universal primers were positive. The sequenced 26 (49%) samples were identified as Sphingomonas spp. being this microorganism the causative agent of this outbreak. In 17% of the cases, patients were reported to have aquatic activities. Conclusion: Several Sphingomonas spp. infections in humans have been reported, mostly limited to sporadic case reports or intra-hospitalary outbreaks, but as far as we know this is the first outbreak of Sphingomonas spp. described in a non-hospital environment. The association between 17% of patients with aquatic activities suggests that this was the most feasible transmission way. Training of health staff and development of new diagnostic able to be implemented in rural endemic areas is urgent in order to overcome wrong diagnostics and avoid wrong treatments.
Autor: Cornejo, A.; Casabona, V.; Gomes, C.S.P.; Tinco, C.; Martinez Pucho, S.; Suarez, L.; Ruiz, J.; Del Valle Mendoza, Juana
Fuente: 16th ICID Abstracts / International Journal of Infectious Diseases 21S (2014)
URL: http://hdl.handle.net/10757/347058
Evaluation of three PCR schemes for detection of Bartonella bacilliformis in blood samples: sensitivity, specificity and applicability
Abstract
Background: Bartonella bacilliformis is the etiological agent of Carrion’s disease, a neglected illness with a febrile lethal stage and a warty benign phase, being the human the only known reservoir. The diagnostic by microscopy in endemic areas is several times erroneous. Furthermore, the culture of this bacterium is time-consuming, being the diagnostic by PCR the easiest way to perform a correct diagnostic. The objective of this study was to evaluate the detection limit of three PCR schemes, designed to detect B.bacilliformis, both in blood and filter papers to test their potential use for transferring samples from endemic areas to reference centers. Moreover, the specificity was also observed as well as the applicability of the technique with clinical samples from different stages of the disease. Methods & Materials: Fragments of 16SrRNA and fla genes were amplified as well as the variable-intergenic region (its). The detection limit was determined by bacterial quantification with flow cytometry and performing dilutions (106cfu/ml-10cfu/ml) both in blood and filter papers. DNA was extracted and PCRs were performed. Specificity was tested by processing other bacteraemia microorganisms. Clinical samples, 12 from febrile patients, 13 from warty and 71 from healthy asymptomatic individuals living in endemic area(Mandinga-Cajamarca) were also processed. Results: The 16SrRNA PCR scheme showed the lower detection limit (5 cfu from blood and filter paper) being the PCR scheme chosen to be tested in clinical samples. All febrile patients’ samples were positive, whereas in warty individuals only 3(23%) faint bands were obtained. No amplification was obtained in samples from healthy people. Fainter bands were always obtained when PCRs were made of filter papers. All PCRs were specific for B.bacilliformis. Conclusion: The 16SrRNA PCR seems to be the best technique to detect feverish patients. However, the applicability to identify asymptomatic carriers was undetermined. Filter paper may be an alternative for easy transportation of samples but is need to consider the decreasing sensitivity of the results. It is critical to develop rapid, sensitive and specific technique capable of being applied in endemic rural areas, to avoid misdiagnosis and facilitate the detection of asymptomatic carriers that will allow progress towards the eradication of this disease.
Autores: Gomes, C.S.P.; Silva, W.; Tinco, C.; Martinez Puchol, S.; Pons, M.J.; Bazan, J.; Del Valle Mendoza, Juana; Ruiz, J.
Fuente: 16th ICID Abstracts / International Journal of Infectious Diseases 21S (2014)
URL: http://hdl.handle.net/10757/347086
Background: Bartonella bacilliformis is the etiological agent of Carrion’s disease, a neglected illness with a febrile lethal stage and a warty benign phase, being the human the only known reservoir. The diagnostic by microscopy in endemic areas is several times erroneous. Furthermore, the culture of this bacterium is time-consuming, being the diagnostic by PCR the easiest way to perform a correct diagnostic. The objective of this study was to evaluate the detection limit of three PCR schemes, designed to detect B.bacilliformis, both in blood and filter papers to test their potential use for transferring samples from endemic areas to reference centers. Moreover, the specificity was also observed as well as the applicability of the technique with clinical samples from different stages of the disease. Methods & Materials: Fragments of 16SrRNA and fla genes were amplified as well as the variable-intergenic region (its). The detection limit was determined by bacterial quantification with flow cytometry and performing dilutions (106cfu/ml-10cfu/ml) both in blood and filter papers. DNA was extracted and PCRs were performed. Specificity was tested by processing other bacteraemia microorganisms. Clinical samples, 12 from febrile patients, 13 from warty and 71 from healthy asymptomatic individuals living in endemic area(Mandinga-Cajamarca) were also processed. Results: The 16SrRNA PCR scheme showed the lower detection limit (5 cfu from blood and filter paper) being the PCR scheme chosen to be tested in clinical samples. All febrile patients’ samples were positive, whereas in warty individuals only 3(23%) faint bands were obtained. No amplification was obtained in samples from healthy people. Fainter bands were always obtained when PCRs were made of filter papers. All PCRs were specific for B.bacilliformis. Conclusion: The 16SrRNA PCR seems to be the best technique to detect feverish patients. However, the applicability to identify asymptomatic carriers was undetermined. Filter paper may be an alternative for easy transportation of samples but is need to consider the decreasing sensitivity of the results. It is critical to develop rapid, sensitive and specific technique capable of being applied in endemic rural areas, to avoid misdiagnosis and facilitate the detection of asymptomatic carriers that will allow progress towards the eradication of this disease.
Autores: Gomes, C.S.P.; Silva, W.; Tinco, C.; Martinez Puchol, S.; Pons, M.J.; Bazan, J.; Del Valle Mendoza, Juana; Ruiz, J.
Fuente: 16th ICID Abstracts / International Journal of Infectious Diseases 21S (2014)
URL: http://hdl.handle.net/10757/347086
Propiedades psicométricas del cuestionario de autoregulación emocional adaptado para el Perú (ERQP) / Psychometric propierties of the emotional self-regulation questionaire adapted for Peru (ERQP)
Resumen
Se estudió las propiedades psicométricas del Cuestionario de Autorregulación Emocional (ERQ, Gross & Thompson, 2003) adaptado para el Perú (ERQP). Este cuestionario evalúa dos estrategias de regulación emocional: la reevaluación cognitiva y la supresión. El ERQP fue aplicado a una muestra de 320 estudiantes universitarios de ambos sexos de dos universidades privadas. Luego de realizar la adaptación lingüística del cuestionario se estudió la validez de constructo del instrumento. El Análisis Factorial Confirmatorio demostró la estructura bifactorial de la escala, y se comprobó la validez convergente y divergente del cuestionario. El índice de consistencia interna alfa de Cronbach de ambas escalas fue 0.72 para reevaluación cognitiva y 0.74 la supresión. Se discute la importancia de contar con un instrumento que evalúe diversas estrategias de autorregulación emocional dada la importancia de este fenómeno psicológico para la vida de las personas.
Abstract
Se estudió las propiedades psicométricas del Cuestionario de Autorregulación Emocional (ERQ, Gross & Thompson, 2003) adaptado para el Perú (ERQP). Este cuestionario evalúa dos estrategias de regulación emocional: la reevaluación cognitiva y la supresión. El ERQP fue aplicado a una muestra de 320 estudiantes universitarios de ambos sexos de dos universidades privadas. Luego de realizar la adaptación lingüística del cuestionario se estudió la validez de constructo del instrumento. El Análisis Factorial Confirmatorio demostró la estructura bifactorial de la escala, y se comprobó la validez convergente y divergente del cuestionario. El índice de consistencia interna alfa de Cronbach de ambas escalas fue 0.72 para reevaluación cognitiva y 0.74 la supresión. Se discute la importancia de contar con un instrumento que evalúe diversas estrategias de autorregulación emocional dada la importancia de este fenómeno psicológico para la vida de las personas.
Autor: Gargurevich, Rafael; Matos, Lennia
Fuente: Revista de Psicologia
Se estudió las propiedades psicométricas del Cuestionario de Autorregulación Emocional (ERQ, Gross & Thompson, 2003) adaptado para el Perú (ERQP). Este cuestionario evalúa dos estrategias de regulación emocional: la reevaluación cognitiva y la supresión. El ERQP fue aplicado a una muestra de 320 estudiantes universitarios de ambos sexos de dos universidades privadas. Luego de realizar la adaptación lingüística del cuestionario se estudió la validez de constructo del instrumento. El Análisis Factorial Confirmatorio demostró la estructura bifactorial de la escala, y se comprobó la validez convergente y divergente del cuestionario. El índice de consistencia interna alfa de Cronbach de ambas escalas fue 0.72 para reevaluación cognitiva y 0.74 la supresión. Se discute la importancia de contar con un instrumento que evalúe diversas estrategias de autorregulación emocional dada la importancia de este fenómeno psicológico para la vida de las personas.
Abstract
Se estudió las propiedades psicométricas del Cuestionario de Autorregulación Emocional (ERQ, Gross & Thompson, 2003) adaptado para el Perú (ERQP). Este cuestionario evalúa dos estrategias de regulación emocional: la reevaluación cognitiva y la supresión. El ERQP fue aplicado a una muestra de 320 estudiantes universitarios de ambos sexos de dos universidades privadas. Luego de realizar la adaptación lingüística del cuestionario se estudió la validez de constructo del instrumento. El Análisis Factorial Confirmatorio demostró la estructura bifactorial de la escala, y se comprobó la validez convergente y divergente del cuestionario. El índice de consistencia interna alfa de Cronbach de ambas escalas fue 0.72 para reevaluación cognitiva y 0.74 la supresión. Se discute la importancia de contar con un instrumento que evalúe diversas estrategias de autorregulación emocional dada la importancia de este fenómeno psicológico para la vida de las personas.
Autor: Gargurevich, Rafael; Matos, Lennia
Fuente: Revista de Psicologia
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